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prime DX®
Precision Individualized Medicine

Prime DX® is an advanced assay for comprehensive molecular tumor profiling, compatible with both tumor tissue (F FPE ) and blood based liquid biopsy (plasma) .

In today’s era of precision medicine, understanding the genetic alterations that drive cancer is essential for selecting the most appropriate treatment strategy.

prime DX® testing provides clinically actionable insights that help physicians identify potential therapeutic options tailored to each patient’s tumor biology, including:

  • Targeted therapies
  • Immunotherapy
  • Chemotherapy

By delivering a broad molecular profile from a single test, prime DX® supports informed, individualized treatment decisions across multiple therapeutic pathways.  

 

prime DX® provides broad molecular profiling via a 1021 gene NGS (Next Generation Sequencing) panel 1021 panel combined with exome RNA sequencing, enabling comprehensive tumor characterization  .

prime DX® enables simultaneous assessment of multiple clinically relevant biomarkers:

Immunotherapy Biomarkers

» Tumor Mutational Burden (TMB)
» Microsatellite Instability (MSI)
» HLA zygosity

PARP inhibitor–relevant biomarkers

» Genomic instability analysis (GIS)

Immunohistochemistry (IHC) biomarkers

» PD-L1
» HER2
» FRα
» CLDN18.2
» c-MET

Tumor agnostic biomarkers

» NTRK1/2/3 fusions
» RET fusions
» BRAF V600E mutation
» HER2 amplification

prime DX® now combines comprehensive DNA analysis (1021 genes) with exome RNA sequencing targeted capture, enabling a more complete and clinically meaningful characterization of tumor profiles.

DNA sequencing identifies single nucleotide variants (SNVs), insertions/deletions (indels), copy number variations (CNVs), and selected structural rearrangements, while RNA sequencing enables the direct detection of expressed gene fusions and splicing events.

This integrated approach significantly enhances the sensitivity and specificity of fusion detection, particularly in cases where genomic breakpoints are complex, intronic, or not adequately covered by DNA-based methods.

prime DX® test is recommended in the following cases:

  • Tumors with no standard treatment available
  • Patients with advanced solid tumors
  • Second-line or post-line treatment
  • Rare Tumors
  • Tumors of unknown primary origin
  • Tumors with many available treatment options, where the physician must clarify the most effective one based on the individual tumor profile of each patient.

Also,

  • For the identification of immune response biomarkers, to predict if an immunotherapy plan would be a suitable plan or/and to create an efficient immunotherapy plan.

prime DX® & RediScore® NGS for Ovarian, Breast, Pancreatic and Prostate Cancer

With the integration of Homologous Recombination Deficiency (HRD) analysis into clinical decision-making, physicians can:

    • Refine treatment selection and personalize therapy beyond BRCA mutation results.
    • Predict therapeutic response to DNA-damaging agents and PARP inhibitors.
    • Improve patient outcomes through more precise and informed treatment strategies.
    • HRD testing serves as an important complementary diagnostic tool, supporting
      precision oncology in cancer care.

The new prime DX® test offers HRD analysis through both the detection of BRCA1/2 or other HRR gene mutations, as well as the assessment of genomic instability (Rediscore®).

This comprehensive NGS approach provides an integrated evaluation of both Comprehensive Genomic Profiling (CGP) and HRD status in a single assay.

It represents a significant advancement compared to most available tests that focus solely on HRD assessment, and contributes to a more complete molecular characterization of the tumor.

HR Genes analyzed

ATMATRATRXBAP1BARD1BLMBRCA1BRCA2BRIP1CDK12
CHEK1CHEK2C11orf30ERCC1FAM175AFANCAFANCCFANCD2FANCEFANCF
FANCGFANCLFANCMMRE11NBNPALB2RAD50RAD51RAD51BRAD51C
RAD51DRAD52RAD54LRECQLRECQL4WRN

Broader Applications

Although the use of HRD (Homologous Recombination Deficiency) as a biomarker is most well established in
ovarian cancer, it is increasingly being applied across a broader range of tumor types, including:

• Breast cancer
• Pancreatic cancer
• Prostate cancer

HRD assessment supports the selection of targeted therapeutic strategies,
enhancing the implementation of precision oncology across multiple indications.

 

Note:
Additional tumor types may be evaluated upon initial request.

The advantage of combined DNA/RNA analysis

By focusing on the expressed transcriptome, RNA sequencing confirms the functional relevance of detected alterations, reducing false-positive and false-negative results and enhancing confidence in clinical interpretation.

The assay targets coding regions across more than 20,000 genes and over 69,000 transcripts, including partial coverage of UTR regions, ensuring broad and accurate detection of clinically relevant gene fusions.

The RNA component is specifically optimized for challenging clinical samples, such as FFPE-derived material, ensuring robust performance in routine diagnostic settings where sample quality may be limited.

It maintains high performance even with low-input or degraded RNA, delivering reliable and reproducible results.

RNA analysis through prime DX® enables high-sensitivity detection of
gene fusions and transcriptomic alterations, supporting both
therapeutic decision-making and accurate diagnosis.

The addition of RNA sequencing provides important advantages in patient management:

  • Enables detection of known, novel, and rare fusion transcripts with diagnostic and therapeutic relevance
  • Enhances sensitivity for clinically actionable alterations guiding targeted therapies, including
    ALK, NTRK, RET, ROS1, FGFR2/3
  • Supports precise classification of tumors driven by gene rearrangements, particularly in sarcomas
  • Identifies RNA-level events such as alternative splicing and exon skipping variants, including EGFRvIII and MET exon 14 skipping

Our Technology & Expertise

State-of-the-Art Testing Platforms: Utilizing advanced Next-Generation Sequencing (NGS) technologies, in combination with
commercially available and proprietary bioinformatics tools, our integrated testing approach ensures accurate, reliable, and comprehensive data.

 

Experienced Scientific Team: Our team consists of specialized molecular scientists who translate genomic data into clinically actionable insights, supporting and optimizing personalized therapeutic approaches.

 

Two Perspectives, One Powerful Profile: Combining tissue and liquid biopsy for precision care.

 

The prime DX® assay is performed in a CAP-accredited laboratory, while the analysis of selected genes and biomarkers falls within the scope of accreditation according to ISO 15189:2022.

 

Next-Generation Sequencing (NGS) is performed using the MGI DNBSEQ-T7 and MGI DNBSEQ-G400 platforms, which, along with the reagents used, are CE-IVD, ensuring high sensitivity and specificity in multi-sample processing.

  • High volume and quality of information
  • Reduced turnaround time
  • Optimized cost

RESULTS REPORT

The results of the prime DX® analysis include a detailed explanation of all findings, including the following information:
  • Molecular profiling of the tumor tissue with details on specific gene mutations and interactive access to information related to the specific gene

  • Recommended approved therapies that can be of great benefit to the specific patient with interactive access to clinical studies

  • Suggested approved cancer therapies that can be of great benefit to the specific patient with interactive access to clinical studies

  • Suggested investigational cancer therapies with interactive access to clinical studies

  • Treatments with associated resistance that will not be of benefit to the specific patient

  • Medication suggestions with documented indications

Accreditations & Certifications

Genekor ensures a high level of quality, reliability, and security through internationally recognized accreditations and certifications.

 

  • Clinical Reliability: CAP-accredited laboratory and accredited according to ISO 15189:2022 (Cert. No. 822)*, ensuring high standards of clinical accuracy.
  • Data Security: Certified according to ISO/IEC 27001:2022 for Information Security Management, ensuring the protection of sensitive data.
  • Quality Management: Certified quality management system according to ISO 9001:2015, ensuring continuous improvement of processes and services.
  • Method Reliability: The prime DX® test is performed using CE-IVD reagents and data analysis and classification software, in compliance with the IVDR Regulation.

 

*Within the official scope of accreditation.

Frequently Asked Questions
Physicians

Which patients are eligible for the test?

All patients with a solid tumor are eligible.

How long does it take to get my results?

15 business days

What kind of sample and how much is required for the test?

For Prime DX® analysis, an FFPE tumor block from the most recent surgical procedure is required, with a tumor content above 20%, excluding bone samples or biopsies with 3–5 cores.
Either the entire block or four 3 μm sections on positively charged slides (air-dried at room temperature, not baked, flat, without folds), as well as six additional unstained 10 μm sections, may be submitted.

Why is it necessary to analyse so many genes?

As an increasing number of targeted therapies are directed toward specific gene alterations detected in a small percentage of patients, the use of an expanded multi-gene panel generates a large volume of high-value clinical information. This significantly increases the likelihood of identifying a personalized therapeutic plan for each patient.

In addition, it has been demonstrated that the higher the number of genes analyzed, the more reliable the calculation of TMB (Tumor Mutational Burden).

In cases where there is not enough tissue or the tissue that is present does not have good quality DNA and/or RNA:

In cases where there is not enough tissue or the tissue that is present does not have good quality DNA and/or RNA, the prime DX® Liquid test is recommended:

The prime DX Liquid® test also consists of a 1021 gene panel and includes the evaluation of immunotherapy biomarkers such as MSI, TMB, and HLA, enabling the treating physician to design an effective treatment plan for the patient, including immunotherapy, chemotherapy, PARP inhibitors, and assessment of eligibility for clinical trials.

Is a rebiopsy recommended or a material from a previous biopsy can be suitable?

Whenever possible, the use of recent tissue or a new biopsy (re-biopsy) is recommended.
Tissue from an older biopsy is considered unsuitable in the following situations:

  • After targeted therapy

  • After chemotherapy or immunotherapy

  • When long periods of time have passed since tissue collection

  • When the tumor has relapsed or progressed metastatically

When new tissue cannot be obtained, or the available tissue is insufficient in quantity or quality, Prime DX® Liquid is recommended.

Why should I sign the consent form?

GeneKor Medical S.A. follows certified quality and information security systems, which require the written consent of each patient for the use of their genetic material for diagnostic testing.
In addition, written consent is mandatory according to current data protection regulations.

Are there any special transportation conditions?

The sample must be kept at room temperature (25°C).
During the summer period, we recommend including an ice pack in the kit.
The ice pack must not come into direct contact with the samples or accompanying documents.

For specific guidelines for the collection of the prime DX Liquid® blood sample click here for more information.

How do I make the payment for the test?

Our customer service department will provide you with a unique e-banking payment code, or alternatively, payment can be made via credit/debit card or bank transfer.

Is the test covered by any public/private insurance?

For information regarding cost coverage, please contact your insurance provider or our customer service team.

How can I send my sample?

To schedule collection and return of your sample, please contact us.

How will I receive my results?

Your results will be shared with your physician through a secure network, and sent to you via email using a unique, secure access code provided by customer service.

Frequently Asked Questions
Patients

Frequently Asked Questions for Patients

How to order the test?

Our Customer Service Team is committed to answer your questions with regards to the services offered by Genekor. If you would like to order any of the tests that Genekor performs please contact us directly.

*To complete the test, you are required to complete and send the Consent form that you will find on the link below.

If you want to send us your sample, please contact us in order to arrange all procedures.
CONTACT

*For more information on scientific content please contact: scientific.support@genekor.com

Download the promotional brochure here.

*Order in the United Arab Emirates here.